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KMID : 0369820100400060363
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Jorunal of Korean Pharmaceutical Sciences
2010 Volume.40 No. 6 p.363 ~ p.366
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Optimization of Experimental Conditions for In vitro P-glycoprotein Assay Using LLC-GA5 Cells
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Ahn A-Ra
Oh Ju-Hee
Lee Joo-Hyun
Lee Young-Joo
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Abstract
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Identification of compounds that function as P-glycoprotein (P-gp) substrates or inhibitors can facilitate the selection and optimization of new drug candidates. The purpose of this study is to optimize the experimental conditions for in vitro P-gp assay using LLC-GA5 cells, which is a well-known transformant cell line derived by transfecting LLC-PK1 with human MDR1. The amount of rhodamine123 transported by the LLC-GA5 and LLC-PK1 cells was evaluated under the following experimental conditions: 3 different types of transport media, colchicine pretreatment or nontreatment of the cells in the culture media, and with and without poly-L-lysine coating of the culture plates. The assay sensitivity was found to considerably differ depending on the diluents used in the transport media. P-gp-mediated transport in LLC-GA5 cells was most clearly characterized in the Hanks' balanced salt solution based transport media. The sensitivity of P-gp-mediated transport was not changed by colchicine pretreatment or poly-L-lysine coating of the culture plates.
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KEYWORD
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P-glycoprotein, LLC-GA5, Rhodamine 123, Efflux transporter
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